The sample preprocessing stage in proteomics experiments is a critical step to ensure the accuracy and consistency of experimental results. Given the complexity and numerous steps involved in the sample preprocessing workflow, developing an integrated and standardized experimental procedure is crucial for addressing the challenges in proteomics sample preprocessing. The SISPROT^?^ technology launched by BePepBio in 2023 is an integrated proteomics preprocessing technology based on the centrifugal pipette tip "Spintip". It enables one-stop completion of all steps in proteomics sample preprocessing from the crude sample, including protein sample pre-enrichment, reduction, alkylation, enzymatic digestion, and desalting. The processed samples can be directly used for LC-MS/MS analysis. This technology combines high sensitivity, high throughput, and high stability, making it particularly suitable for protein samples in the low microgram or even nanogram range.

Product Features

High Efficiency: Complete sample processing within 2 hours

While traditional methods require 16 hours, the SISPROT Kit completes all sample preprocessing steps within 2 hours, and the processed samples are ready for direct instrumental analysis.

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High Sensitivity: Approximately 6000 proteins detected from 100ng
Approximately 6000 proteins can be stably detected from 100ng. The minimum initial sample amount can be as low as 1ng (equivalent to ~10 cells).

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High Stability: Excellent quantitative reproducibility across different operators

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Popular Products

SISPROT? SE

Stably identifies 7000+ proteins starting from just 500ng of protein, addressing the difficulty in detecting low-abundance proteins. Features simple operation and excellent reproducibility.?Suitable for proteomics sample preprocessing of blood, body fluids, cells, exosomes, and other samples. Applicable in large-scale population cohort studies using blood samples and disease molecular subtyping research, high-throughput screening for drug targets, spatial proteomics, and other fields.

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SISPROT??Flex?

Requires only 1-10μg of protein per operation for conventional samples, saving sample consumption.?Compatible with SDS lysis buffer. Suitable for plant tissue, animal tissue, cells, plasma, serum, saliva, urine, cerebrospinal fluid, protein solutions, and other samples. Applicable in medical research fields such as biological mechanism exploration, diagnostic technology development, and therapeutic strategy research.

Product Information

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Related Literature

? Simple and integrated spintip-based technology applied for deep proteome profiling, Anal. Chem., 2016, 88(9):4864-71.?

??3D-SISPROT: A simple and integrated spintip-based protein digestion and three-dimensional peptide fractionation technology for deep proteome profiling, J. Chromatogr. A, 2017, 1498:207-214.?

? Mixed-mode ion exchange-based integrated proteomics technology for fast and deep plasma proteome profiling, J. Chromatogr. A, 2018, 1564:76-84.

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Shenzhen Baymics Biotechnology Co., Ltd. is an R&D-focused high-tech company specializing in proteomics. Starting with its series of proteomics preprocessing products, the company aims to provide comprehensive workflow solutions for the target market through its clinical functional proteomics technology platform. The company is dedicated to reducing the technical complexity of proteomics analysis and enhancing its application throughout the entire chain of innovative biomarker and drug discovery, providing precise diagnosis and treatment strategies for major diseases using proteomics technology.

As the exclusive China distributor for BePepBio, Beijing Ximeijie Technology Co., Ltd. has established long-term and stable partnerships with numerous well-known domestic pharmaceutical companies and CRO/CMO enterprises. Over the years, Ximeijie's products and services have helped many companies accelerate their R&D stages, improve drug quality, purity, and safety, optimize R&D processes, shorten time-to-market, and reduce QC costs. If you are interested in the aforementioned products, please call the Ximeijie customer service hotline at 400-050-4006 or visit the website?www.emergexcelerate.com?for more information.

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